JOVE JOURNAL

Bioquímica

Showing 673 - 684 in 724 videos.

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Journal (Biochemistry)

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Zheng Ma*¹, Victor Fung*¹, Iván D'Orso

¹Department of Microbiology,The University of Texas Southwestern Medical Center

Journal (Biochemistry)

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Yuno Natsume, Hsin-i Wen, Tong Zhu, Kazumi Itoh, Li Sheng, Kensuke Kurihara^3,4

¹Department of Mathematical and Physical Sciences, Faculty of Science,Japan Women's University, ²Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience,National Institutes of Natural Sciences, ³Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science,National Institutes of Natural Sciences, ⁴Research Center for Complex Systems Biology,The University of Tokyo

Journal (Biochemistry)

Laboratory Scale Production and Purification of a Therapeutic Antibody

Zehra Elgundi, Vicki Sifniotis, Mouhamad Reslan, Esteban Cruz, Veysel Kayser

¹Faculty of Pharmacy,University of Sydney

Journal (Biochemistry)

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto, Hidesato Ogawa, Hiroaki Taniguchi^4,5

¹Graduate School of Frontier Biosciences,Osaka University, ²Department of Biotechnology and Genetic Engineering,Jahangirnagar University, ³Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences,Doshisha University

Journal (Biochemistry)

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Xinzhou Zhu, Andrea Zelmer, Sven Wellmann²

¹University Children's Hospital Basel (UKBB),University of Basel, ²Department of Clinical Research,University of Basel

Journal (Biochemistry)

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Carlos Eduardo Espinosa-de la Garza*¹, Rodolfo Daniel Salazar-Flores*¹, Néstor Octavio Pérez, Luis Francisco Flores-Ortiz, Emilio Medina-Rivero

¹Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

Journal (Biochemistry)

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Gatikrushna Singh*¹, Sarah M. Fritz*², Arnaz Ranji, Deepali Singh, Kathleen Boris-Lawrie²

¹Department of Veterinary & Biomedical Sciences,University of Minnesota, ²Department of Veterinary Biosciences,Ohio State University, ³School of Biotechnology,Gautam Buddha University

Journal (Biochemistry)

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Jonathan Fischer, Roger A. Johnson, Elizabeth Boon

¹Department of Chemistry,Stony Brook University, ²Department of Physiology and Biophysics,Stony Brook University

Journal (Biochemistry)

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Masatake Kanai, Shoji Mano², Mikio Nishimura

¹Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, ²Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), ³Department of Cell Biology,National Institute for Basic Biology

Journal (Biochemistry)

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Ryan W. Clinton, Jason A. Mears

¹Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Journal (Biochemistry)

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Clemens Entzian, Thomas Schubert

¹2bind GmbH

Journal (Biochemistry)

A Rapid Laser Probing Method Facilitates the Non-invasive and Contact-free Determination of Leaf Thermal Properties

Johannes F. Buyel², Hannah M. Gruchow, Martin Wehner

¹Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., ²Institute for Molecular Biotechnology,RWTH Aachen University, ³Fraunhofer Institute for Laser Technology ILT,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

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