JOVE JOURNAL

Bioquímica

Showing 25 - 36 in 724 videos.

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Journal (Biochemistry)

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Designing Streptavidin Affinity Grids for Cryo-Electron Microscopy

Journal (Biochemistry)

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Trinity Cookis, Paul Sauer³, Simon Poepsel⁵, Bong-Gyoon Han, Dominik A. Herbst^2,6, Robert Glaeser, Eva Nogales^2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Sample Preparation for In Situ Cryotomography of Mammalian Cells

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon

Anjelique Sawh-Gopal², Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez, Edward T. Eng, Alex J. Noble

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

Journal (Biochemistry)

Using Smart Leginon Autoscreen for CryoEM Grid Screening

Anjelique Sawh-Gopal², Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez, Edward T. Eng, Alex J. Noble

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

Journal (Biochemistry)

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Ayaka Hagita-Tatsumoto², Tomohiro Miyasaka^2,3

¹Department of Neuropathology, Faculty of Life and Medical Sciences,Doshisha University, ²Center for Research in Neurodegenerative Diseases,Doshisha University, ³Laboratory of Physiology and Anatomy, School of Pharmacy,Nihon University

Journal (Biochemistry)

TEV Protease-Mediated Elution and Denaturation Elution of the Chromatin Locus Purified from Saccharomyces cerevisiae

Atiqa Sajid, Stephan Hamperl

¹Institute of Epigenetics and Stem Cells,Helmholtz Zentrum München

Journal (Biochemistry)

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae

Atiqa Sajid, Stephan Hamperl

¹Institute of Epigenetics and Stem Cells,Helmholtz Zentrum München

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