JOVE JOURNAL

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Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Taylor C. Brown, Norman G. Nicolson, Joyce Cheng, Reju Korah, Tobias Carling

¹Department of Surgery & Yale Endocrine Neoplasia Laboratory,Yale University School of Medicine, ²Yale New Haven Hospital

Journal (Cancer Research)

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Thomas Boyer², Fanny Gonzales³, Adriana Plesa, Pauline Peyrouze, Adeline Barthelemy, Soizic Guihard, Bruno Quesnel⁵, Christophe Roumier², Claude Preudhomme², Meyling Cheok

¹Laboratoire d’hématologie,CHU Lille, ²Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, ³Service d’hématologie pédiatrique, hôpital Jeanne de Flandre,CHU Lille, ⁴Laboratoire d’hématologie CH Lyon Sud, ⁵Service des Maladies du Sang, hôpital Claude Huriez,CHU Lille

Journal (Cancer Research)

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

Kenji Amemiya², Yosuke Hirotsu, Toshio Oyama, Masao Omata³

¹Genome Analysis Center,Yamanashi Central Hospital, ²Pathology Division, Laboratory Department,Yamanashi Central Hospital, ³The University of Tokyo

Journal (Cancer Research)

In Vivo EPR Assessment of pH, pO₂, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Andrey A. Bobko², Timothy D. Eubank³, Benoit Driesschaert², Valery V. Khramtsov²

¹In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center,West Virginia University, ²Department of Biochemistry,West Virginia University School of Medicine, ³Department of Microbiology, Immunology & Cell Biology,West Virginia University School of Medicine

Journal (Cancer Research)

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Alessia Volpe, Francis Man, Lindsay Lim, Alex Khoshnevisan, Julia Blower, Philip J. Blower, Gilbert O. Fruhwirth

¹Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences,King's College London

Journal (Cancer Research)

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Tanner J. McArdle, Brenda M. Ogle^2,3,4,5, Felicite K. Noubissi

¹Department of Biomedical Engineering,University of Minnesota - Twin Cities, ²Stem Cell Institute,University of Minnesota - Twin Cities, ³Masonic Cancer Center,University of Minnesota - Twin Cities, ⁴Lillehei Heart Institute,University of Minnesota - Twin Cities, ⁵Institute for Engineering in Medicine,University of Minnesota - Twin Cities, ⁶Department of Biology,Jackson State University

Journal (Cancer Research)

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Michael M. Lizardo², Poul H. Sorensen³

¹Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health, ²BC Cancer Agency,Provincial Health Services Authority, ³Department of Pathology and Laboratory Medicine,University of British Columbia

Journal (Cancer Research)

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Jonathan F. Anker, Hanlin Mok, Anum F. Naseem, Praveen Thumbikat*^1,3, Sarki A. Abdulkadir*^1,2,3

¹Department of Urology,Northwestern University Feinberg School of Medicine, ²The Robert H. Lurie Comprehensive Cancer Center,Northwestern University Feinberg School of Medicine, ³Department of Pathology,Northwestern University Feinberg School of Medicine

Journal (Cancer Research)

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Jacqueline Cloos*^1,2, Jeffrey R. Harris*³, Jeroen J.W.M. Janssen, Angele Kelder, F. Huang, Gerrit Sijm, Maike Vonk, Alexander N. Snel, Jennifer R. Scheick, Willemijn J. Scholten, Jannemieke Carbaat-Ham, Dennis Veldhuizen, Diana Hanekamp, Yvonne J.M. Oussoren-Brockhoff, Gertjan J.L. Kaspers⁴, Gerrit J. Schuurhuis, A. Kate Sasser, Gert Ossenkoppele

¹Department of Hematology,VU University Medical Center, ²Pediatric Oncology/Hematology,VU University Medical Center, ³Janssen Research & Development, LLC, ⁴Princess Máxima Center for Pediatric Oncology

Journal (Cancer Research)

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Andrew K. Fuller², Benjamin D. Bice², Ashlee R. Venancio², Olivia M. Crowley², Ambur M. Staab², Stephanie J. Georges², Julio R. Hidalgo², Annika V. Warncke², Melinda L. Angus-Hill²

¹Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,University of Utah, ²Huntsman Cancer Institute,University of Utah

Journal (Cancer Research)

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

Jing Huang*^1,2, Peng Guo*^1,2, Marsha A. Moses²

¹Vascular Biology Program,Boston Children's Hospital, ²Department of Surgery,Harvard Medical School and Boston Children's Hospital

Journal (Cancer Research)

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1⁺ Tim-3⁺ CD8⁺ T Cells

Clémence Granier, Emeline Vinatier^3,4, Elia Colin³, Marion Mandavit³, Charles Dariane^3,5, Virginie Verkarre, Lucie Biard, Rami El Zein, Corinne Lesaffre, Isabelle Galy-Fauroux, Hélène Roussel^3,6, Eléonore De Guillebon, Charlotte Blanc³, Antonin Saldmann, Cécile Badoual⁶, Alain Gey*⁴, Éric Tartour*^2,4

¹PARCC-INSERM U970,Université Paris Descartes, ²INSERM U970,Université Paris Descartes, ³Equipe Labellisée Ligue Contre le Cancer, ⁴Department of Immunology,Hopital Européen Georges Pompidou, ⁵Department of Medical Urology,Hopital Européen Georges Pompidou, ⁶Department of Pathology,Hopital Européen Georges Pompidou, ⁷Université Paris Diderot Paris 7, ⁸Department of medical oncology,Hopital Européen Georges Pompidou

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