All Protocols and Video Articles for scientific research | JoVE

Showing 49 - 60 of 108 videos for "EcoRI"

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Journal (Bioengineering)

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Victoria Messerschmidt*¹, Zachary Bailey*¹, Kyung In Baek, Yichen Ding, Jeffrey J. Hsu, Richard Bryant, Rongsong Li, Tzung K. Hsiai, Juhyun Lee

¹Department of Bioengineering,The University of Texas at Arlington, ²Department of Medicine (Cardiology) and Bioengineering,UCLA, ³College of Health Science and Environmental Engineering,Shenzhen Technology University

Journal (Genetics)

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Seoghyun Lee*¹, Minho Won*², Ran Hee Hwang, Gang Min Hur, Hyunju Ro

¹Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, ²Department of Pharmacology, College of Medicine,Chungnam National University, ³Department of Nursing,Gwangju Women's University

Journal (Genetics)

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Stefanie L. Morgan*^1,2, Erin Y. Chang*¹, Natasha C. Mariano, Abel Bermudez, Nicole L. Arruda, Fanting Wu, Yunhai Luo, Gautam Shankar, Star K. Huynh, Chiao-Chain Huang, Sharon J. Pitteri, Kevin C. Wang^2,6

¹Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, ²Program in Cancer Biology,Stanford University School of Medicine, ³Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, ⁴Department of Biology,Bridgewater State University, ⁵System Biosciences, ⁶Veterans Affairs Healthcare System

Journal (Developmental Biology)

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Weijun Feng*¹, Lena Herbst*², Peter Lichter, Stefan M. Pfister^4,5, Hai-Kun Liu, Daisuke Kawauchi⁴

¹Division of Molecular Neurogenetics,German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, ²Division of Molecular Genetics,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ³Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), ⁴Division of Pediatric Neurooncology,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ⁵Department of Pediatric Hematology and Oncology,Heidelberg University Hospital

Journal (Neuroscience)

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Agnes Thalhammer, Fanny Jaudon, Lorenzo A. Cingolani

¹Center for Synaptic Neuroscience and Technology,Istituto Italiano di Tecnologia

Journal (Cancer Research)

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay

Ying Wang, DanQi Chen, MingYu Chen, KaiYuan Ji, DeXuan Ma, LiangFu Zhou

¹Department of Neurosurgery, Huashan Hospital,Fudan University

Journal (Biochemistry)

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

April Maschmann, Cody Masters, Melissa Davison, Joshua Lallman, Drew Thompson, Kristy L. Kounovsky-Shafer

¹Department of Chemistry,University of Nebraska - Kearney

Journal (Neuroscience)

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Long Mei², Cheng Zhan, Eric Erquan Zhang

¹PTN Joint Graduate Program, School of Life Sciences,Peking University, ²National Institute of Biological Sciences, Beijing

Journal (Biology)

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Wan-Shan Yang, Mel Campbell, Hsing-Jien Kung^3,4,5, Pei-Ching Chang⁶

¹Institute of Microbiology and Immunology,National Yang-Ming University, ²UC Davis Cancer Center,University of California, Davis, ³Department of Biochemistry and Molecular Medicine,University of California, Davis, ⁴Institute for Translational Medicine, College of Medical Science and Technology,Taipei Medical University, ⁵Division of Molecular and Genomic Medicine,National Health Research Institutes, ⁶Center for Infectious Disease and Cancer Research,Kaohsiung Medical University

Journal (Biochemistry)

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Smarajit Polley², De-Bin Huang, Tapan Biswas, Gourisankar Ghosh

¹Department of Chemistry & Biochemistry,University of California, San Diego, ²Department of Biophysics,Bose Institute

Journal (Bioengineering)

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Alessandra Merenda², Amanda Andersson-Rolf², Roxana C. Mustata, Taibo Li, Hyunki Kim, Bon-Kyoung Koo²

¹Wellcome Trust - Medical Research Council Stem Cell Institute,University of Cambridge, ²Department of Genetics,University of Cambridge, ³Department of Pathology,Yonsei University College of Medicine

Journal (Genetics)

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Erin L. Sternburg, Kristen C. Dias, Fedor V. Karginov

¹Cell Biology and Neuroscience,University of California, Riverside

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