All Protocols and Video Articles for scientific research

Showing 1 - 12 of 36 videos for "Glucose DMEM"

Production of Recombinant AAV by Transient Transfection

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC - Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC - Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC,University of Coimbra

Journal (Medicine)

Cell Culture and Cellular Thermal Shift Assay (CETSA) Sample Preparation for Western Blotting

Liuling Luo, Jinrui Xiong, Yancai Tang, Xiaorui Chen, Hai Zhang

¹State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine

Journal (Biology)

Murine Lymphatic Endothelial Cell Purification

Racheal Grace Akwii*¹, Margarita Lamprou*², Maria Georgomanoli, Andriana Plevriti, Antonia Marazioti, Athanasia Mouzaki, George Mattheolabakis, Constantinos M. Mikelis²

¹Department of Pharmaceutical Sciences, School of Pharmacy,Texas Tech University Health Sciences Center, ²Laboratory of Molecular Pharmacology, Department of Pharmacy,University of Patras, ³Division of Flow Cytometry and Division of MDx & Life Sciences,SB BioAnalytica, ⁴Basic Sciences Laboratory, Department of Physiotherapy,University of Peloponnese, ⁵Division of Hematology, Department of Internal Medicine, Medical School,University of Patras, ⁶School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy,University of Louisiana at Monroe

Journal (Immunology and Infection)

Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

Daniel Call*¹, Sabrina S. Pizarro*^2,3, Erica Tovey, Emily Knight³, Carrie Baumgardner⁴, Kenneth A. Christensen, James C. Morris

¹Department Chemistry and Biochemistry,Brigham Young University, ²Eukaryotic Pathogens Innovation Center,Clemson University, ³Department of Genetics and Biochemistry,Clemson University, ⁴Department of Physics and Astronomy,Clemson University

Journal (Immunology and Infection)

Intracellular Replication Assay to Examine the Molecular Pathogenesis of Shigella

Kender Poore*¹, Bryan R. Lenneman*^1,2, Christina S. Faherty²

¹Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, ²Department of Pediatrics,Harvard Medical School

Journal (Developmental Biology)

Tissue Studies by Culturing Reconstituted Chicken Skin

Tingxin Jiang, Maeve Secor, Rusty Lansford, Randall B. Widelitz, Cheng Ming Chuong

¹Department of Pathology,Keck School of Medicine of University of Southern California, ²Molecular and Computational Biology,University of Southern California, ³Department of Radiology,Keck School of Medicine of University of Southern California

Journal (Developmental Biology)

Chicken Skin Epithelial-Mesenchymal Recombination Culture

Tingxin Jiang, Maeve Secor, Rusty Lansford, Randall B. Widelitz, Cheng Ming Chuong

Journal (Developmental Biology)

Chicken Skin Explant Culture for Organogenesis Study

Tingxin Jiang, Maeve Secor, Rusty Lansford, Randall B. Widelitz, Cheng Ming Chuong

Journal (Developmental Biology)

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Karin Tourle, Amber Rucinski, Alexander Grainger, Ioannis J. Limnios, Macarena Gonzalez Ruiz, Jonathan K.H. Tan

¹Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine,Bond University

Journal (Bioengineering)

Methods Collections

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Ana Luísa Graça, Niklas Kroner-Weigl, Viviana Reyes Alcaraz, Sigrid Müller-Deubert, Maximilian Rudert, Denitsa Docheva

¹Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg, ²Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery,University Hospital Regensburg, ³Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg

Journal (Biology)

Methods Collections

Optimizing Mouse Primary Lens Epithelial Cell Culture: A Comprehensive Guide to Trypsinization

Yu Yu*¹, Jinmin Zhang*¹, Hongli Wu²

¹Pharmaceutical Sciences, College of Pharmacy,University of North Texas Health Science Center, ²North Texas Eye Research Institute,University of North Texas Health Science Center

Journal (Neuroscience)

Methods Collections

Two-Photon Polymerization 3D-Printing of Micro-scale Neuronal Cell Culture Devices

Ali Hosseini, Giovanni Noselli, Michele Giugliano

¹Neuroscience Department,International School for Advanced Studies, ²Mathematics Department,International School for Advanced Studies

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