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Summary
Abstract
Protocol
Discussion
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Biologia

录像示范保存鼠气味追踪，但经过伞 - 穹窿病变受损的惯性导航试验

Published: April 24, 2009
doi:
10.3791/1193
,
1Department of Neuroscience,Canadian Centre for Behavioural Neuroscience, University of Lethbridge

Summary

在试点的气味跟踪任务，返回食物的庇护所使用的视觉气味线索或使用红外光的航迹推算能力的大鼠，以前运动的综合记录，显示，海马航迹推算是必要的。

Abstract

试点和航位推算导航策略使用的非常不同的线索星座和计算过程（1873年，达尔文;巴洛，1964年;奥基夫和迟•那德利，1978年;米特尔施泰特和米特尔施泰特，1980年; Landeau等，1984;艾蒂安，1987; Gallistel， 1990年，毛雷尔和Séguinot，1995年）。试点需要使用相对稳定的外部线索（视觉，嗅觉，听觉）之间的关系，而航迹推算需要整合的自我运动所产生的线索。动物前庭感受器的自我运动信息的获取，并可能是肌肉和关节的受体，并efference产生运动的命令的副本。动物也可使用流动的视觉，听觉和嗅觉刺激的运动引起的。使用一个试点战略的动物可以用几何计算确定的方向和距离，在其环境中的地方，而使用航位推算策略，它能够整合其先前的动作所产生的线索，回到刚刚离开的位置。航迹推算是俗称“方向感”和“距离感”。

虽然有相当多的证据表明，海马是参与试点（奥基夫和迟•那德利，1978年;奥基夫和斯皮克曼，1987年），也有从行为（Whishaw等，1997年的证据。Whishaw Maaswinkel，1998年; Maaswinkel和Whishaw，1999），建模（Samsonovich及McNaughton，1997年），和电（O'Mare等人，1994年，夏普等，1995;陶布和Burton，1995年，布莱尔和夏普，1996年，麦克诺顿等1996年维也纳，1996年;葛洛布和陶布，1997年），海马的形成是在参与航迹推算的研究。海马的相对贡献导航两种形式仍是未知数，但是。按说，这是因为动物是在其战略和线索（艾蒂安等，1996使用非常灵活。Dudchenko等，1997; Martin等难以确定的动物使用的是与航位推算战略试点，1997; Maaswinkel和Whishaw，1999年）。本视频演示的目的是解决问题的提示规范，为了研究海马在使用这些策略的相对贡献。在新任务中，他们遵循直线或多边形的香味步道获得一家大型食品颗粒隐藏在一个开放的领域，各组大鼠进行了培训。因为老鼠有一个倾向携带食物回的避难，准确性和用于返回到大本营（Whishaw和富江，1997年）因变量的线索。要强制一个动物AA死推算战略到同食其避难，对大鼠进行测试时被蒙住眼睛或红外光下，一个光谱波长中，他们可以不看到，在一些实验的气味线索是另外删除一旦动物达到食物。为了研究海马伞 – 穹窿（FF）的病变，从而破坏在海马结构的信息流（平淡，1986年）的相对贡献，损害记忆“（Gaffan和Gaffan，1991），和生产的空间赤字（Whishaw和Jarrard， 1995年），分别采用。

Protocol

动物在室温保持在20-21℃，12小时照明实验室，12个成年女性长 – 埃文斯大鼠（莱斯布里奇动植物公园大学），体重250-300公克，被安置在铁丝网笼子组光/暗周期（上午8点至晚上8时）。 6只收到深水业务和6前收到的测试伞 – 穹窿病变。 外科无菌手术，将大鼠麻醉与钠苯巴比妥（40毫克/公斤，IP）和阿托品甲基硝酸盐（5毫克/公斤，IP）。为…

Discussion

实验研究利用在大鼠challanged向外的行程，从他们的避难所，位于字符串的结尾的食物颗粒后香味字符串后返回家园的一项新任务的海马与航位推算导航试点的贡献。对照组大鼠导航有效地使用空间和航迹推算策略。伞 – 穹窿病变大鼠成功地利用空间的战略，但均受损时被迫使用航位推算。这些结果与假设航迹推算的海马结构的完整性至关重要一致。这也可能是在跟踪一个比狗等动物的第一个示范…

Acknowledgements

这项工作是由加拿大健康研究学会的支持。

Materials

Material Name Tipo Company Catalogue Number Comment
Sodium pentobarbital Reagent Sigma-Aldrich p3761-25g  
Atropine methyl nitrate Reagent Sigma-Aldrich a0382-5g  
Rodent pellets Animal food BIO-SERV    
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Tags

Preserved PilotingScent TrackingImpaired Dead ReckoningFimbria-fornix LesionsRat Navigation StrategiesCue ConstellationsComputational ProcessesPiloting CuesDead Reckoning CuesExternal CuesSelf-movement InformationVestibular ReceptorsEfference CopyVisual StimuliAuditory StimuliOlfactory StimuliGeometrical CalculationsHippocampus Involvement In Piloting
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Whishaw, I. Q., Gorny, B. P. A Video Demonstration of Preserved Piloting by Scent Tracking but Impaired Dead Reckoning After Fimbria-Fornix Lesions in the Rat. J. Vis. Exp. (26), e1193, doi:10.3791/1193 (2009).
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