The growth media (YPD) and synthetic dropout media (SD) including LRK and LHRK, and enriched sporulation media used in this protocol is routinely used in yeast molecular biology. Please refer to Methods in Yeast (Amberg et al., 2005) for detailed descriptions.
Tavassoli, S., Chao, J. T., Loewen, C. A high-throughput method to globally study the organelle morphology in S. cerevisiae. J. Vis. Exp. (25), e1224, doi:10.3791/1224 (2009).