This video demonstrates how to measure cell invasion through cell culture inserts using a fluorescence-based methodology.
The authors have nothing to disclose.
Material Name | Tipo | Company | Catalogue Number | Comment |
---|---|---|---|---|
BD BioCoat Tumor Invasion System | BD Biosciences | 354165 or 354166 | ||
HT-1080 and NIH/3T3 cells | ATCC | |||
Dulbecco’s Modified Eagle Medium (DMEM; serum-free) | ||||
Dulbecco’s Phosphate Buffered Saline, without calcium and magnesium (DPBS) | ||||
BD Falcon FluoroBlok 24-Multiwell Insert System to be used as a cell migration control | BD Biosciences | 351157 or 351158 | ||
5% Fetal Bovine Serum in DMEM | ||||
BD Calcein AM Fluorescent Dye | BD Biosciences | 354216 or 354217 | ||
Hanks’ Balanced Salt Solution (HBSS) | ||||
BD Falcon 24-well plates for post cell invasion labeling | BD Biosciences | 351147 | ||
Fluorescence plate reader with bottom reading capabilities | PerkinElmer EnVision (R), TECAN Infinite (R), Molecular Devices SpectraMax (R), BioTek Synergy (TM), BMG LABTECH PHERAstar. |