This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.
This video article demonstrates how to use the MXcell electroporation system to easily identify optimal electroporation conditions for MEFs or other primary cell lines. The 96-well plate format allows for many replicates of experimental or optimization conditions to be performed simultaneously, which can eliminate the need for many separate experiments. While carrying out this procedure, one should to remember to use healthy cells as soon after isolation as possible and to use electroporation conditions that are matched to the electroporation buffer.
The authors have nothing to disclose.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Gene Pulser® Electroporation Buffer | Bio-Rad Laboratories, Inc. | 165-2676 | ||
Gene Pulser MXcell™ Electroporation System | Bio-Rad Laboratories, Inc. | 165-2670 | ||
Gene Pulser MXcell™ ShockPod™ Cuvette Chamber | Bio-Rad Laboratories, Inc. | 165-2673 | ||
Gene Pulser MXcell™ Electroporation System With ShockPod™ Cuvette Chamber | Bio-Rad Laboratories, Inc. | 165-2674 |