יצירה וטיהור של ספריית לכידת קונפורמציה של כרומוזומים (3C)

Published: May 12, 2023

doi:

10.3791/200093

Acadia Joniec*¹, Joseph Leszczynski*¹, Sokhna Ndoye*¹, Jillian Sylvia*¹, Steven E. Weicksel²

¹Department of Biological and Biomedical Sciences,Bryant University, ²Center of Health and Behavioral Sciences,Bryant University

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Keywords: Chromosome Conformation Capture 3C Library Caenorhabditis Elegans Chromatin Cross-linking Nuclei Collection DpnII Restriction Enzyme Chromatin Digestion Chromatin Ligation T4 DNA Ligase Protein Digestion Reverse Cross-linking Proteinase K RNase A Phenol-chloroform Extraction Purification

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Generation and Purification of Chromosome Conformation Capture (3C) Library. J. Vis. Exp. (Pending Publication), e200093, doi:10.3791/200093 (2023).

יצירה וטיהור של ספריית לכידת קונפורמציה של כרומוזומים (3C)

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