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인간 중간엽 줄기 세포를 5리터에서 15리터 바이오리액터로 2계층 확장 및 15리터 바이오리액터에서 자동화된 세포 제형

Published: July 07, 2023

doi:

Yuyang Chen*¹, Huanye Xu*², Yuanyuan Zhang*², Leilei Guo, Miaomiao Lan, Yanping Yang, Wei Liu, Xiaojun Yan, Yanan Du

¹Department of Biomedical Engineering, School of Medicine,Tsinghua University, ²Beijing CytoNiche Biotechnology Co. Ltd.

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Mesenchymal Stem Cells Bioreactor Cell Expansion Automated Cell Formulation Cell Harvest Cell Processing Microcaririer Serum-free Medium Cell Inoculation Medium Supplement Medium Exchange Cell Sampling Cell Harvest Cell Suspension Cell Washing Cell Culture

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Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor. J. Vis. Exp. (Pending Publication), e200420, doi:10.3791/200420 (2023).

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