Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34<sup>+</sup> Stem Cells for Cardiovascular Studies

doi:10.3791/201053

JoVE Journal > Medicine

Medicina

Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34⁺ Stem Cells for Cardiovascular Studies

Published: December 22, 2023

doi:

10.3791/201053

Chuting Han, Cheng Xie, Qingya Dang, Xiaolin Zhang, Chunshu Li, Yan Yang, Jun Cheng, Pengyun Li

¹Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research,Southwest Medical University

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Immunomagnetic Isolation Vascular Wall-resident CD34+ Stem Cells Purification Flow Cytometry Functional Identification Immunofluorescence Staining Ca2+ Imaging Murine Aorta And Mesenteric Artery Adventitia Cell Culture Multipotency Differentiation ATP Caffeine Thapsigargin

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Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34⁺ Stem Cells for Cardiovascular Studies. J. Vis. Exp. (Pending Publication), e201053, doi:10.3791/201053 (2023).

Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34+ Stem Cells for Cardiovascular Studies

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Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34⁺ Stem Cells for Cardiovascular Studies