Nematode Slide Preparation: A Method to Mount Animals on an Agar Pad
Published: April 30, 2023
Abstract
Source: Teoh, J. S., et al. Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans. J. Vis. Exp. (2019).
During live imaging of C. elegans, an agarose pad can be used to hold the nematode still without damaging or drying it. This video describes how to prepare a simple mounting pad for microscopy.
Protocol
This protocol is excerpted from Teoh et al, Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans, J. Vis. Exp. (2019). Preparation of slides for imaging Prepare 3-4% w/v agarose stock in a microwave-safe bottle (3-4 g in 100 mL of ultrapure water). NOTE:</strong…
Materials
| Agar-agar | Merck | 1.01614.1000 | |
| Agarose | Invitrogen | 16500-500 | |
| Glass coverslips #1 | Thermo scientifique | MENCS22221GP | |
| Glass coverslips #1.5 | Zeiss | 474030-9000-000 | Made by SCHOTT |
| Glass slides | Thermo scientifique | MENS41104A/40 | |
| Pasteur pipette | Corning | CLS7095D5X-200EA | |
| Platinum wire | Sigma | 267201-2G | |
| Tetramisole hydrochloride | Sigma | L9756-5G |
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Nematode Slide Preparation: A Method to Mount Animals on an Agar Pad. J. Vis. Exp. (Pending Publication), e20132, doi: (2023).