Hindlimb Skeletal Muscle Extraction: A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model
Published: April 30, 2023
Abstract
Source: Bonetto, A. et al. The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia. J. Vis. Exp. (2016)
In this video, we describe the procedure to excise hindlimb skeletal muscles from a mouse model. The excised muscles can be stored at lower temperatures until further downstream analysis.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Tissue and Organ Excision NOTE: For use of tissues in biochemical or molecular biology assays, plan to weigh each organ and tissue and place a fragment immediately into pre-labeled cryotubes. Snap freeze in liquid nitrogen and store at -80 °C. In order to avoid contamina…
Declarações
The authors have nothing to disclose.
Materials
| CD2F1 mice | Harlan | 60 | |
| Anesthesia apparatus | EZ-Anesthesia | EZ-7000 | |
| OCT | Tissue-Tek | 4583 | |
| Cryostat | Leica | CM1850 | |
| Cork disks | Electron Microscopy Sciences | 63305 | |
| Microtainer | BD | 365873 | |
| Superfrost plus glass slides | VWR | 48311-703 | |
| Streptomycin-Penicillin | Cellgro | 30-002-CI |
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Hindlimb Skeletal Muscle Extraction: A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model. J. Vis. Exp. (Pending Publication), e20422, doi: (2023).