In this video, we demonstrate the preparation of a decellularized pig esophagus scaffold, with its biochemical architecture and composition preserved, for tissue engineering applications.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Decellularized Esophageal Scaffold Obtain intact porcine esophagi at an abattoir from freshly slaughtered Landrace pigs that are used for food production. One single esophagus will provide sufficient scaffolds for approximately 30 separate constructs. NOTE: Due to the extensive and…
Declarações
The authors have nothing to disclose.
Materials
DMEM
Labtech
LM-D1112
Warm in 37 °C water bath before use
Ham's F12
Labtech
LM-H1236
Warm in 37 °C water bath before use
Foetal Calf Serum
Labtech
FB-1090
Epidermal Growth Factor
R+D Systems
236-EG-200
Prepare 200 µg/ml stock solution in 10 mM acetic acid, 1% FCS
Cholera toxin
Sigma-Aldrich
C8052
Prepare stock solution in water
L-Glutamine
Sigma-Aldrich
G7513
Penicillin-Streptomycin
Sigma-Aldrich
P0781
Amphotericin B
Gibco
15290-026
Brand name Fungizone
PBS
Oxoid
BR0014
Dissolve 1 tablet in 100 ml water and autoclave to sterilize
Decellularized Porcine Esophageal Scaffold Preparation: A Technique to Generate Acellular Scaffold from a Pig Esophagus. J. Vis. Exp. (Pending Publication), e20494, doi: (2023).