Retinal Cup Extraction from Mouse: A Surgical Procedure to Obtain Intact Retinal Cup from Mouse Eye

Abstract

Source: Jiang, K. et al. Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples. J. Vis. Exp (2021)

This video describes a protocol to isolate the retinal cup without the outer covering layers from the eyeball of a mouse. The extracted retinal tissue can help study various metabolic pathways occurring in the retinal cells.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Retinal cup dissection Euthanize a mouse by CO2 asphyxiation following institutional guidelines on euthanasia. NOTE: Do not leave the animal in a CO2 chamber longer than the time needed for euthanasia. Enucleate eyes and place into ice-old 1x PBS buffer …

Materials

1X PBS	Thermo Fisher	14190-144
30-gauge needle	BD Precision Glide	305106
Biopsy puncher, 1 mm	Integra Miltex	33-31AA
CO2 asphyxiation chamber
Dissection forceps-Dumont #5	Fine Science Tools	11251-10	Stright tip
Dissection forceps-Dumont #7	Fine Science Tools	11274-20	Curved tip
Dissection microscope
Graefe forceps	Fine Science Tools	11051-10	Curved, Serrated tip
Microscissors	Fine Science Tools	15004-08	Curved tip