All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Brain staining and slicing
Figure 1: Matrices for the rat heart and brain slicing. (A) Rat heart, (B) rat brain.
2,3,5-Triphenyltetrazolium chloride (TTC) | Sigma-Aldrich | 298-96-4 | |
Adult Rat Brain Slicer Matrix | Zivic Instruments | BSRAS001-1 | |
Single Edge Razor Blades | Zivic Instruments | BLADE012.1 | |
Sony Alpha a6000 Mirrorless Digital Camera | Sony | ILCE6000 | Can be repalaced by any up-to-date digiatal camera |
Surgical blade | Heinz Herenz Hamburg Germany | BS2982 | |
Weigh tray, 70 mL | Sarsted | 71,99,23,212 | |
Thermo-Shaker | BioSan | PST-60HL-4 | |
Toothed tissue forceps | Agnthos | 11021-12 | |
Sodium chloride | Fisher bioreagents | BP358-10 | |
Cover Glass Forceps, Angled | Fine Science Tools | 11073-10 |
Source: Liepinsh, E., et al. Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion. J. Vis. Exp. (2022)
This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.
Source: Liepinsh, E., et al. Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion. J. Vis. Exp. (2022)
This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.
Source: Liepinsh, E., et al. Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion. J. Vis. Exp. (2022)
This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.