Isolation of Marginating Hepatic Leukocytes from a Mouse Liver

Abstract

Source: Sorski, L., et al. Selective Harvesting of Marginating-hepatic Leukocytes. J. Vis. Exp. (2016)

In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Mouse Protocol

1. Perfusion of the Liver and Collection of MH-Leukocytes
   1. Euthanize the animal with an overdose of 8% isoflurane. As a precaution, place a 15 ml tube containing an isoflurane-soaked pad around the mouse's head until opening its chest cavity.
   2. Fix the limbs of the mouse to a bed.
   3. Open the peritoneal and chest cavities to expose the liver and the cardiopulmonary complex immediately upon cessation of respirat....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Autoclud Peristaltic pump
Butterfly needle	OMG		26G
Butterfly needle	OMG		21G*3/4"
Syringe	Pic solution		1 ml
Syringe	Pic solution		2.5 ml
Syringe	Pic solution		5 ml
Syringe	Pic solution		10 ml
Syringe	Pic solution		25 ml
Blunted-edged forceps
Scissors
Hemostat
Tissue forceps