Protokoll für Mosquito Aufzucht (A. gambiae)
Summary
Dieses Video zeigt den allgemeinen Techniken verwendet, um hinten Anopheles gambiae im Labor. Die Methoden für die Pflege Labor Mücken sind in allen Phasen des Organismus Lebenszyklus von Larven zu Blut-Fütterung Erwachsene Puppen gezeigt.
Abstract
Dieses Protokoll beschreibt Mücke Aufzucht im Insektarium. Das Insektarium Zimmer sind bei 28 ° C und ca. 80% Luftfeuchtigkeit, mit einem 12-Std. Tag / Nacht-Zyklus. Für dieses Verfahren benötigen Sie Moskito-Käfige, 10% sterile Saccharose-Lösung, Papierhandtücher, Becher, Whatman-Filterpapier, Glas Speiser, menschlichem Blut und Serum, Wasserbad, Parafilm, destilliertes Wasser, saubere Kunststoff-Trays, Moskito Essen (beschrieben unten), Moskitonetz, um die Fächer, Vakuum, und eine Sammlung Kammerdeckel zu Erwachsenen zu sammeln.
Protocol
Materials
| Material Name | Tipo | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Food A | Aquaricare | Grounded fish food. A small pinch needs to be added. | ||
| Food B | Purina | Grounded cat food. A small pinch needs to be added. | ||
| Food C | Purina | Cat food. Two tablets needs to be added | ||
| RBC | prepared red blood cells: 40% hematocrit in human serum | |||
| RPMI | Medium | |||
| CPD | anticoagulant | |||
| serum | O+ human serum | |||
| Mosquito feeder | ||||
| parafilm | ||||
| Anopheles gambiae | mosquitos | |||
| 10% sucrose | mosquito food | |||
| insectary |
Referências
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