This video demonstrates the development of a 3D co-culture system. Mouse brain slices are placed on a membrane, and fluorescently labeled human brain tumor cells are allowed to grow on it. Once the system is ready, it is stained for imaging.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Plating the Slices onto the Inserts Fill each well of a 6-well plate with 1,200 µl of slice culture media. Fill any unused wells with 1,200 µl PBS and fill the center space of the plate with 5 ml 1x PBS. Store this plate at 37 °C. Using the slotted spoon tool, place a brain slice into the media on the insert, gently pushi…