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Opprette Definerte Gassformige Miljøer å studere effekter av hypoksi på<em> C. elegans</em
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JoVE Journal Biology
Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

Opprette Definerte Gassformige Miljøer å studere effekter av hypoksi på<em> C. elegans</em

DOI:
10.3791/4088-v

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11:07 min

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July 20, 2012

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, ,
1Department of Biochemistry,University of Washington, 2Molecular and Cellular Biology Program,University of Washington

Chapters

  • 00:05Title
  • 01:27Construction of Environmental Chambers
  • 02:56Connecting the Gas Supply to the Environmental Chamber
  • 04:39Preparation of Samples for Viability Assay
  • 07:32Rapid Harvest of Hypoxia-exposed Worms
  • 10:11Results: The Effect of Hypoxia on C. elegans
  • 10:44Conclusion

Summary

Automatic Translation

Automatic Translation

Notatet informasjon om hvordan å bruke kontinuerlig flyt hypoksi kamre for å generere atmosfærer med definerte konsentrasjoner av O<sub> 2</sub> Å forstå biologiske responser til redusert O<sub> 2</sub>. Dette systemet er enkelt å installere og vedlikeholde, og fleksibel nok til å dekke et bredt spekter av O<sub> 2</sub> Konsentrasjoner og modellere systemer

Tags

Defined Gaseous EnvironmentsHypoxiaC. ElegansCellular Signaling PathwaysTreatment StrategiesModel OrganismGenetically AmenableHypoxic ResponseO2 ConcentrationsTranscriptional ResponseHif-1Ppm O2

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