A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:35Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR
- 02:48In Vitro Transcription
- 03:59Treatment of Purified mRNA with Antartic Phosphatase
- 05:17mRNA Transfection of Cells
- 07:01Flow Cytometric Analyses of eGFP Expression in Cells
- 08:14Results: Modified mRNA is Expressed in Human Cells
- 09:34Conclusion
