动电位腐蚀试验
Summary
Here, we present a protocol to set up and run an in vitro potentiodynamic corrosion system to analyze pitting corrosion for small metallic medical devices.
Abstract
Different metallic materials have different polarization characteristics as dictated by the open circuit potential, breakdown potential, and passivation potential of the material. The detection of these electrochemical parameters identifies the corrosion factors of a material. A reliable and well-functioning corrosion system is required to achieve this.
Corrosion of the samples was achieved via a potentiodynamic polarization technique employing a three-electrode configuration, consisting of reference, counter, and working electrodes. Prior to commencement a baseline potential is obtained. Following the stabilization of the corrosion potential (Ecorr), the applied potential is ramped at a slow rate in the positive direction relative to the reference electrode. The working electrode was a stainless steel screw. The reference electrode was a standard Ag/AgCl. The counter electrode used was a platinum mesh. Having a reliable and well-functioning in vitro corrosion system to test biomaterials provides an in-expensive technique that allows for the systematic characterization of the material by determining the breakdown potential, to further understand the material’s response to corrosion. The goal of the protocol is to set up and run an in vitro potentiodynamic corrosion system to analyze pitting corrosion for small metallic medical devices.
Introduction
电化学技术提供了快速且相对便宜的方法获得的材料的电化学性能。这些技术在通过观察电荷转移过程的受控电化学干扰1-5的响应，以检测金属的腐蚀的能力，主要是基础的。体的环境中的金属植入物的腐蚀是非常关键的，由于在生物相容性和材料完整性6的不利影响。促进身体内植入物的腐蚀的主要因素是表面氧化，导致金属离子7-11的释放增多的溶解。这将导致不良的生物反应，其可以发现局部的，但具有潜在的全身效应，导致植入物10,12-28的过早失效。
试样的腐蚀特性从产生的偏振扫描预测由恒电位。偏振扫描允许对金属基板的动能和腐蚀参数的推断。在扫描期间，氧化或还原的电活性物种可以通过电荷转移和反应物或产物的运动受到限制。这些因素都是由偏振扫描包封;因此具有在多个周期产生一个可靠和可重复的偏振扫描系统的重要性是非常重要的。这份手稿的重点是提供一个确定的协议，以获得一个运作良好的电位腐蚀体系所采取的基本原理和步骤。
Protocol
Representative Results
Discussion
从不锈钢试样产生偏振扫描显示清洁连续曲线，在指示运作良好的腐蚀系统，该系统既可靠和可再现29的文献看出扫描相关联。差电位点蚀电位的再现性是确定与几百毫伏的蔓延，与孔蚀电位的特征在于一个随机过程29。这通常是由于温度，卤化物含量和电势（V）的变量;因此，从实际安装在E 科尔获得较小的变化是指示协议，并具有改善体外设置上述调整。
<p cla…Declarações
The authors have nothing to disclose.
Acknowledgements
The authors had no funding provided for this study.
Materials
| Potentiostat | Metrohm | PGSTAT101 | |
| Ag/AgCl reference electrode, shielded | Metrohm | 6.0729.100 | |
| Electrode shaft | Metrohm | 6.1241.060 | |
| Polisher Forcipol 1v | Metkon | 3602 | |
| Clindrical flask 700mL | SciLabware | FR700F | |
| Reaction lid | SciLabware | MAF2/41 | |
| Dichloromethane | Sigma-Aldrich | MKBR7629V | use under a fumehood. Wear protective clothing |
| Thermo / HAAKE D Series Immersion Circulators | Haake |
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