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线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

JoVE Journal
Biologia

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JoVE Journal Biologia

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

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线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

DOI:

10.3791/55166-v

•

08:43 min

•

January 24, 2017

•

Matthew McKenzie², Sze C. Lim, Michael R. Duchen

¹Centre for Genetic Diseases,Hudson Institute of Medical Research, ²The Department of Molecular and Translational Sciences,Monash University, ³Department of Physiology,University College London

Capítulos

00:05Título
00:54Preparation of Cells
02:21Staining of Cells with TMRM and Fluo-4 AM
03:46Cell Imaging and Analysis
06:44Results: Measuremnt of Mitochondrial Membrane Potential and Calcium in Digitonin Permeabilized 143B Cells
07:57Conclusion

Summary

Tadução automática

线粒体可以利用在其内的膜（Δ _膜电势）的电化学势螯合钙（Ca ^2+），允许它们塑造细胞内的Ca ²⁺的细胞内信号传导。我们描述了使用荧光染料和共聚焦显微镜同时测量线粒体的Ca ²⁺摄取和ΔΨ _微米活细胞的方法。

Tags

Mitochondrial Calcium Mitochondrial Membrane Potential Fluorescent Microscopy Live Cell Imaging Calcium Regulation Mitochondrial Disease Calcium-free HBSS TMRM Fluo 4 AM Digitonin Thapsigargin

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