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一个协议来使用基因集富集分析以确定合适的动物模型转化型研究

JoVE Journal
Biologia

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JoVE Journal Biologia

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

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一个协议来使用基因集富集分析以确定合适的动物模型转化型研究

DOI:

10.3791/55768-v

•

09:35 min

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August 16, 2017

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Christopher Weidner, Matthias Steinfath, Elisa Wistorf, Michael Oelgeschläger, Marlon R. Schneider, Gilbert Schönfelder²

¹Department of Experimental Toxicology and ZEBET,German Federal Institute for Risk Assessment (BfR), ²Department of Clinical Pharmacology and Toxicology,Charité-Universitätsmedizin Berlin

Capítulos

00:05Título
00:54Performing the Game Set Enrichment Analysis (GSEA)
05:46Comparing the GSEA Results and Identifying the Optimal Animal Model
07:55Results: Overlap of Pathway Regulation Identifies Suitable and Unsuitable Animal Models
08:49Conclusion

Summary

Tadução automática

我们提供一个标准化的协议用于基因集的浓缩转录组数据分析，找出理想的小鼠模型的转化研究。
该协议可以用 DNA 微阵列和 RNA 测序数据，并且可以进一步扩展到其他组学数据如果数据可用。

Tags

Gene Set Enrichment Analysis Animal Model Translational Research Genome Data Mouse Models Inflammatory Diseases Gene Expression Studies Gene Expression Data Phenotype File Molecular Signature Database DNA Chip Annotations GSEA Software Gene Symbols Permutations

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