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En protokoll for bruk av Gene satt berikelse analyse for å identifisere den aktuelle dyr modellen for translasjonsforskning
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JoVE Journal Biology
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

En protokoll for bruk av Gene satt berikelse analyse for å identifisere den aktuelle dyr modellen for translasjonsforskning

DOI:
10.3791/55768-v

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09:35 min

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August 16, 2017

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, , , , ,
1Department of Experimental Toxicology and ZEBET,German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology,Charité-Universitätsmedizin Berlin

Chapters

  • 00:05Title
  • 00:54Performing the Game Set Enrichment Analysis (GSEA)
  • 05:46Comparing the GSEA Results and Identifying the Optimal Animal Model
  • 07:55Results: Overlap of Pathway Regulation Identifies Suitable and Unsuitable Animal Models
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Vi gir en standardisert protokoll for bruk av genet angi berikelse analyse av transcriptomic data til å identifisere en ideell musemodell for translasjonsforskning.
Denne protokollen kan brukes med microarray DNA og RNA sekvensering data og kan videre utvides til andre omics data hvis data er tilgjengelige.

Tags

Gene Set Enrichment AnalysisAnimal ModelTranslational ResearchGenome DataMouse ModelsInflammatory DiseasesGene Expression StudiesGene Expression DataPhenotype FileMolecular Signature DatabaseDNA Chip AnnotationsGSEA SoftwareGene SymbolsPermutations

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