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Imunologia e Infecção

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基于磁珠复合法的撕裂细胞因子分析

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

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基于磁珠复合法的撕裂细胞因子分析

DOI:

10.3791/55993-v

•

09:22 min

•

October 13, 2017

•

Praveen Kumar Balne², Veonice Bijin AU, Louis Tong, Arkasubhra Ghosh, Mukesh Agrawal, John Connolly, Rupesh Agrawal

¹National Healthcare Group Eye Institute,Tan Tock Seng Hospital, ²Singapore Eye Research Institute (SERI), ³Institute of Molecular and Cell Biology,A*STAR, ⁴GROW Research Laboratory,Narayana Nethralaya Foundation, ⁵Vimta Labs Limited

Capítulos

00:05Título
00:42Elution of Tear Sample from Tear Flow Strip
01:26Cytokine Profiling by Bead Based Multiplex Assay
04:43Reading the Assay Plate and Data Analysis
06:56Results: Tear Cytokine Profiles from Four Male Subjects
08:54Conclusion

Summary

Tadução automática

泪膜细胞因子的分析有助于研究各种眼部疾病。基于微珠的复合检测方法简单、灵敏, 能够对小体积样品中的多个目标进行测试。在这里, 我们描述了一个协议的撕裂膜细胞因子分析一个使用珠基复用法。

Tags

Tear Cytokine Profile Bead-based Multiplex Assay Schirmer Strip Ophthalmology Cytokines Ocular Diseases Analytes Tear Sample Assay Buffer Centrifugation Cytokine Profiling Multiplex Assay Cytokine Standards Assay Worksheet Magnetic Plate Washer

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