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高脂膳食喂养和高通量三测定在果蝇中的含量

JoVE Journal
Genética

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JoVE Journal Genética

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

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高脂膳食喂养和高通量三测定在果蝇中的含量

DOI:

10.3791/56029-v

•

08:28 min

•

September 13, 2017

•

Soda Balla Diop, Ryan T. Birse, Rolf Bodmer

¹Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute

Capítulos

00:05Título
01:08High Fat Diet Preparation
02:15High Fat Diet Feeding
03:32Weighing the Flies
04:22Grinding the Flies
05:50Sample Loading and TAG Content Determination
06:54Results: Changes in Fat Content after High Fat Diet Feeding
07:39Conclusion

Summary

Tadução automática

这是一个高脂肪饮食喂养协议, 以诱发肥胖在果蝇, 一个模型, 了解的基本分子机制牵连脂。它还提供了一种高通量三测定方法, 用于测量在各种饮食、环境、遗传或生理条件下的果蝇中的脂肪积累和潜在的其他 (昆虫) 模型。

Tags

High Fat Diet High Throughput Triacylglyceride Assay Drosophila Melanogaster Obesity Metabolism Lipotoxicity Diabetes Metabolic Syndrome Liver Steatosis Cardiac Steatosis Drug Discovery High Fat Food Coconut Oil Food Preparation Fly Food Anesthesia Aging High Fat Diet Feeding Normal Food

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