Målet med denne studien var å bestemme effekten av blekemiddel og klor gass-sterilisering på frø spiring av et utvalg av Arabidopsis genotyper dyrket på sterilt media. Optimalisert sterilisering protokollene er utviklet for å hindre vekst av mikrobielle forurensninger samtidig som tilfredsstillende frø overlevelse.
Arabidopsis thaliana (Arabidopsis) planter trenger ofte å dyrkes på sterilt media. Dette krever forutgående frø sterilisering å hindre vekst av mikrobielle forurensninger til stede på frø overflaten. Foreløpig er Arabidopsis frø sterilisert med to forskjellige sterilisering teknikkene i forhold som varierer noe mellom laboratorier og ikke er standardisert, ofte resulterer i bare delvis Effektiv sterilisering eller overdreven frø dødelighet. De fleste av disse metodene er heller ikke lett skalerbar til et stort antall frø linjer med ulike genotyper. Som teknologi for høy gjennomstrømming analyse av Arabidopsis fortsette å spre, blir standardisert teknikker for sterilisering stort antall frø av ulike genotyper avgjørende for å gjennomføre disse typer eksperimenter. Responsen av Arabidopsis linjer på to forskjellige sterilisering teknikker ble evaluert basert på frø spiring rate og nivået av frø forurensning med mikrober og andre patogener. Behandlinger inkludert ulike konsentrasjoner av sterilisering agenter og ganger eksponering, kombinert for å bestemme optimale forhold for Arabidopsis frø sterilisering. Optimalisert protokoller har blitt utviklet for to forskjellige sterilisering metoder: blekemiddel (flytende-fase) og (Cl2) klorgass (damp-fase), både som resulterer i høy frø spiring priser og minimal mikrobiell forurensning. Nytten av disse protokollene ble illustrert gjennom testing av både wild type og mutant frø med en rekke spiring potensialer. Våre resultater viser at frø kan være effektivt sterilisert bruke enten metoden uten overdreven frø dødelighet, selv om skadevirkninger sterilisering ble observert i frø med lavere enn optimalt spiring potensial. I tillegg ble en formel utviklet for å muliggjøre forskere bruke standardiserte klor gass sterilisering betingelsene lufttette beholdere av ulike størrelser. Protokollene beskrevet her tillate enkel, effektiv og rimelig frø sterilisering for en rekke Arabidopsis linjer.
Arabidopsis thaliana (Arabidopsis) er en førsteklasses modell organisme for grunnleggende anvendt forskning i anlegget biologi1,2,3. Mens standard betingelser for Arabidopsis vekst har vært godt etablert4, har effekten av frø sterilisering på frø levedyktighet ikke blitt grundig testet. Solid media i plater eller boksene er rutinemessig brukes til å muliggjøre vekst av Arabidopsis frøplanter for mange eksperimentelle programmer, som homozygous dødelige mutanter i en segregerende befolkning, observasjon av skyte og rot fenotyper på tidlig etapper, isolering av patogen-fri vev, samling av store mengder frøplante vev, transformants eller narkotika-resistente planter og evaluering spiring1,2,3,4 . Frø høstet fra planter dyrket i drivhus eller vekst kammer er noen ganger forurenset med mikroorganismer og støv. Vekst av Arabidopsis frøplanter på forskjellige medietyper sterilt krever forutgående frø sterilisering å fjerne mikrobiell forurensninger som sopp og bakterier på frø overflaten. Bruk av en effektiv frø sterilisering regime er avgjørende for en balanse mellom høy spiring, minimum forurensning og frodig plantevekst.
De to vanligste metodene brukes for Arabidopsis frø sterilisering er basert på kommersielle blekemiddel (flytende-fase) og klorgass (damp-fase). Ulike prosedyrer har vært ansatt både væske-fase sterilisering1,4,5,6,7,8,9 , 10 og damp-fase sterilisering av Arabidopsis frø8,10,11,12,13,14,15 ,16. Men mens disse prosedyrene er effektive i å oppnå frø sterilisering av utnyttet genotyper, er en detaljert analyse av effekten av ulike sterilisering behandlinger på frø av ulike genotyper ikke rapportert. Derfor er optimalisering av disse dampsterilisering nødvendig å definere betingelser som Effektiv sterilisering kombinert med høy spiring rate.
The Arabidopsis biologiske Resource Center (ABRC) ligger unikt til a) test frø levedyktigheten til en rekke ulike genotyper i samlingen og b) dra nytte av kvalitetskontroll prosedyrer brukes internt og som svar på tilbakemeldinger om frø spiring. Målene for eksperimenter presenteres her var å bestemme effekten av ulike sterilisering på frø spiring av et utvalg av Arabidopsis genotyper. Optimalisert dampsterilisering som resulterer i høy frø spiring priser samtidig opprettholde minimale patogen forurensning er presentert for både blekemiddel og klor gass-sterilisering.
Når økende Arabidopsis frø på sterilt media, må noen form for sterilisering brukes. Både blekemiddel og klor gass sterilisering behandlinger resultere i lignende spiring rate og mold vekst hemming. Verken steriliserings metode fører til en betydelig reduksjon i spiring sats for frø med høy spiring potensielle; bleach sterilisering anbefales imidlertid for linjer med lavere spiring potensial (20-70%), på grunn av lille, men ikke betydning, forbedring i spiring rate i forhold til gass-sterilisering (figur 3A).
Sterilisering av Arabidopsis frø med blekemiddel konsentrasjoner fra 40-100% for opptil 10 min gir tilfredsstillende spiring prosenter og effektiv mold undertrykkelse. Selv om blekemiddel konsentrasjoner mindre enn 40% gi tilstrekkelig sterilisering for de fleste frø mye, med en konsentrasjon av 40% eller høyere garanterer effektiv sterilisering med tungt forurenset frø masse. Det er viktig å ikke overskride 10 min sterilisering ved blekemiddel konsentrasjoner lik eller høyere enn 80% å unngå høy frø dødelighet og feil i frøplante utvikling.
Behandling av Arabidopsis frø med klor konsentrasjoner på 6,1% eller 16,5% 1 h resultat i høy spiring priser og tilstrekkelig mold eliminering. Lav klor gass konsentrasjon (2,1%) kan brukes med hell ved å øke varigheten av sterilisering 3t.
Når linjene må steriliseres, anbefales flytende sterilisering i en løsning av 50% blekemiddel i 10 min. For større antall linjer er gass-sterilisering med en gass konsentrasjon på 6,1% 1t et bedre alternativ siden mange linjer kan steriliseres raskt og enkelt med mindre manipulering.
Våre resultater gir standardiserte forholdene for sterilisering både stort antall frø av ulike genotyper og frøene med lavere spiring potensielle. Den eneste begrensningen for disse sterilisering teknikker er at de ikke kan brukes med frø med spiring priser mindre enn 20% på grunn av omfattende frø dødelighet. Alternative metoder, for eksempel sonication17, å øke spiring rate i fravær av sterilisering kan være nyttig i disse tilfellene.
The authors have nothing to disclose.
Vi vil gjerne takke Gauri Datta for henne hjelp forberede experimental materialer. Vi er også takknemlige for Bettina Wittler og James Mann for den kritiske gjennomgangen av manuskriptet. Dette arbeidet ble støttet av NSF tilskudd DBI-1049341 og kalt MCB-1143813.
0.65 mL Microcentrifuge tubes | GeneMate | C-3260-5 | Tube in which Arabidopsis thaliana seeds are placed to perform sterilization |
1.7 mL Microcentrifuge tube | GeneMate | C-3262-1 | Tube in which Arabidopsis thaliana seeds are placed to perform sterilization |
7 L plastic container | Sistema | 1016265438 | Container in which gas sterilization is performed |
Concentrated HCl | Sigma-Aldrich | 320331 | Chemical used in the process of creating chlorine gas |
Disposable sterile inoculating loop | Fisher Scientific | 22-363-603 | Loop is used to spread or position Arabidopsis seeds on MS plates |
Gamborg’s vitamin solution | Sigma-Aldrich | G1019 | Vitamin solution used in the process of making MS media |
Household bleach | Clorox | Regular-Bleach2 | Chemical used in the process of creating chlorine gas and liquid sterliziation |
MES hydrate | Sigma-Aldrich | M2933 | Chemical used in the process of making MS media |
Micropore surgical tape | 3M | 1530-1 | Microporous surgical paper tape used to seal MS plates |
Murashige and Skoog basal salt mixture (MS) | Sigma-Aldrich | M5524 | Chemical used in the process of making MS media |
Parafilm M | Bemis Company | #PM996 | Parraffin film used to seal sterilization container |
Petri dish 100 X 15 mm | Fisher Scientific | FB0875713 | Petri dishes in which MS media is poured for the purpose of growing Arabidopsis thaliana |
pH indicator strips | Whatman | 2613991 | Used to check pH of neutralizied chlorine and sodium bicarbonate solution |
Phytoagar | Fisher Scientific | 50-255-212 | Used to aid in the suspension of Arabidopsis seeds in the process of plating seeds |
Sodium bicarbonate | Sigma-Aldrich | S5761 | Chemical used in the process of neutralizing chlorine gas reaction |
Sucrose | Sigma-Aldrich | S0389 | Chemical used in the process of making MS media |
Tween 20 | Fisher BioReagents | BP337-100 | Chemical used in the process of liquid sterilization |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | CS70000 (Col-0) | Arabidopsis wild-type seeds |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_037606C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_041402C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_059101C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_063470C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_072048C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_072240C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_081989C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_084124C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_085049C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_089717C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_107354C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_110111C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_111322C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_113109C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_114702C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_114872C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_115657C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_116803C | Arabidopsis seeds used in testing as a part of Group 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_039445C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_039782C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_043037C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_045828C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_048556C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_049514C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_049725C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_080816C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_081176C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_081770C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_082262C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_082289C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_082702C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_083630C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_084635C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_085337C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_085656C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_093049C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_103332C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_105336C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_105704C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_106388C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_109575C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_110580C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_110617C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_111424C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_111584C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_112097C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_113339C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_115837C | Arabidopsis seeds used in testing as a part of Group 2 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_019535C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_026478C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_046565C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_049258C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_049339C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_056307C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_081292C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_081597C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_083488C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_110573C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_112793C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_113658C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_113904C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_114673C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_114709C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_115455C | Arabidopsis seeds used in testing as a part of Group 3 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_013186C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_018261C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_062509C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_080639C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_088586C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_096651C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_106900C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_110131C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_111051C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_111245C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_113223C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_121391C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_125097C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_201905C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_210001C | Arabidopsis seeds used in testing as a part of Group 4 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_000662C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_029335C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_047760C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_071275C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_080530C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_103881C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_110864C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_120294C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_124390C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_132808C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_137036C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_139519C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_140643C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_142288C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_143304C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_147597C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_209076C | Arabidopsis seeds used in testing as a part of Group 5 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_081081C | Arabidopsis seeds used in testing as a part of Groups 5 and 1 |
Arabidopsis thaliana seeds | ABRC; order through TAIR www.arabidopsis.org | SALK_107487C | Arabidopsis seeds used in testing as a part of Groups 5 and 2 |