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通过检测全小鼠胚胎β乳糖酶活性追踪基因表达

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

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通过检测全小鼠胚胎β乳糖酶活性追踪基因表达

DOI:

10.3791/57785-v

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08:42 min

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June 26, 2018

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María José Blanco*¹, Ana I.R. Learte*¹, Miguel A. Marchena, Emma Muñoz-Sáez, María Antonia Cid, Iván Rodríguez-Martín, Cristina Sánchez-Camacho³

¹Faculty of Biomedical and Health Sciences,Universidad Europea de Madrid, ²Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ³School of Doctoral Studies and Research,Universidad Europea de Madrid

Capítulos

00:04Título
00:49β-galactosidase Histochemistry of Whole Mount Mouse Embryos
02:23Paraffin Embedding of X-gal Stained Embryos
04:37Paraffin Sectioning
05:44Deparaffinization and Counterstaining
06:56Results: X-gal Staining of E9.5 WT and Mt4-mmpLacZ/ Whole-mount Embryos and Tissue Sections
07:52Conclusion

Summary

Tadução automática

在这里, 我们描述了检测早期全小鼠胚胎β乳糖酶活性的标准协议, 以及石蜡切片和 counterstaining 的方法。这是一个简单和快速的过程, 以监测基因表达在发展期间, 也可以应用于组织切片, 器官或培养细胞。

Tags

Î²-galactosidase Gene Expression Whole Mount X-gal Staining Mouse Embryos Biologia do Desenvolvimento Reporter Gene Lineage Tracing Histochemical Detection Paraffin Sectioning

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