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Kvantificere leukocyt udgang via lymfekar fra Murine hud og tumorer
JoVE Journal
Biology
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JoVE Journal Biology
Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors
DOI:

08:39 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:46Mouse Pinna Inflammation Induction, Vaccinia Infection, Photoconversion, and Tissue Processing
  • 02:52Intradermal Melanoma Tumor Photoconversion and Tissue Processing
  • 04:00Flow Cytometry Preparation & Analysis
  • 05:18Results: Representative Photoconversion Efficiency in Skin and the Tumor Microenvironments
  • 07:55Conclusion

Summary

Automatic Translation

Her, viser vi metoder for i vivo kvantificering af leukocyt afstigning fra naiv, betændt, og ondartet murine hud. Vi udføre en head-to-head sammenligning af to modeller: transdermal FITC ansøgning og i situ photoconversion. Derudover viser vi nytte af photoconversion for sporing leukocyt afstigning fra kutane tumorer.

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