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果蝇梅拉诺加斯特的锡酸盐合成酶活性的色度测定

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

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果蝇梅拉诺加斯特的锡酸盐合成酶活性的色度测定

DOI:

10.3791/59454-v

•

04:30 min

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January 16, 2020

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Ping Wei*¹, Qihong Liu*², Wen Xue, Jiwu Wang

¹Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, ²Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Capítulos

00:04Título
00:38Colorimetric Citrate Synthase Activity Assay
02:46Results: Effect of dRNF34 and dPGC-1 Knockdown on Citrate Synthase Activity
03:38Conclusion

Summary

Tadução automática

我们提出了一种对胆酸合成酶活性的色度测定方案，用于定量果蝇组织均质中完整的线粒体质量。

Tags

Citrate Synthase Colorimetric Assay Drosophila Melanogaster Mitochondrial Function Extraction Buffer Protein Concentration Enzymatic Activity DRNF34 DPGC-1

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