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使用纳米-牛顿分辨率力转换器从骨骼肌肉活检中隔离肌异物并确定收缩函数

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer

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使用纳米-牛顿分辨率力转换器从骨骼肌肉活检中隔离肌异物并确定收缩函数

DOI:

10.3791/61002-v

•

07:55 min

•

May 07, 2020

•

Martijn van de Locht, Josine M. de Winter, Dilson E. Rassier, Michiel H.B. Helmes³, Coen A.C. Ottenheijm

¹Department of Physiology,Amsterdam UMC, ²Department of Kinesiology and Physical Education, Faculty of Education,McGill University, ³IONOptix BV

Capítulos

00:04Introduction
00:50Myofibril and Mounting Needle Preparation
02:08Experiment Initialization
04:03Tension Redevelopment Rate Measurement
05:04Stepwise Stretch
05:33Results: Representative Active and Passive Tension Analyses
07:03Conclusion

Summary

Tadução automática

此处介绍的是一个协议，用于评估具有纳米-牛顿分辨率的条纹肌肉肌异半孔的收缩特性。该协议采用基于干涉测量的光学力探头的设置。此设置生成具有高信噪比的数据，并可评估 myofibril 的收缩动力学。

Tags

Myofibrils Skeletal Muscle Biopsies Contractile Function Nano-Newton Resolution Force Transducer Sarcomere Dysfunction Sarcomeric Proteins Permeabilized Cardiomyocytes Homogenization Myofibril Suspension Relaxing Solution Poly-HEMA Coated Slide Shellac Ethanol Glue Mounting Needle Micro-manipulators Sarcomere Length Vessel Function Video Imaging Microscope Stage

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