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1Department of Plant Pathology, Coastal Plain Experiment Station,University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center,University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center,Tennessee State University
Capítulos
- 00:04Introduction
- 00:48Pump and Filter Setup
- 01:16Water Sampling and Filtering
- 02:22Magnetic Bead-Based DNA Extraction
- 03:58Loop-Mediated Isothermal Amplification (LAMP) Assay
- 05:07Results: Representative Phytophthora capsici Detection
- 06:53Conclusion
Nous avons développé une méthode pour détecter phytophthora capsici zoospores dans les sources d’eau à l’aide d’une méthode d’extraction d’ADN de papier filtre couplée à un test d’amplification isothermique (LAMP) à médiation en boucle qui peut être analysé sur le terrain ou en laboratoire.
