Journal
/
/
来自肠道密码的穆林原结肠上皮单层的生成
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
DOI:

06:57 min

February 06, 2021

, , , ,

Capítulos

  • 00:04Introduction
  • 00:51Preparation of Plates, Chamber Slides and Cell Culture Membrane Inserts
  • 01:30Crypt Isolation
  • 04:09Culturing 2D Monolayer
  • 05:17Results: Reliability of The Primary Epithelial Colon Monolayer Cultures
  • 06:17Conclusion

Summary

Tadução automática

在此协议中,我们描述了如何直接从肠道密码生成穆林原发性上皮结肠单层。我们提供实验方法,在透气过滤器上生成汇流单层,在划痕伤口愈合和生化研究中产生汇流单层,以及用于免疫荧光分析的稀疏和共生单层。

Vídeos Relacionados

Read Article