/

/

开发一种细胞共培养模型以模拟心脏缺血/体外再灌注

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

Please note that all translations are automatically generated. Click here for the English version.

开发一种细胞共培养模型以模拟心脏缺血/体外再灌注

DOI:

10.3791/62913-v

•

08:05 min

•

October 13, 2021

•

Zhu Li, Matthew J. W. Hampton, Matthew B. Barajas, Matthias L. Riess^2,3

¹Department of Anesthesiology,Vanderbilt University Medical Center, ²Anesthesiology,TVHS VA Medical Center, ³Department of Pharmacology,Vanderbilt University

Capítulos

00:04Introduction
00:50Experimental Preparation/Plating
02:58Hypoxia/Reoxygenation to Simulate Ischemia/Reperfusion Injury In Vitro
04:28Endpoint Assessment
05:17Results: Effect of Three Types of Culture Inserts and Different Concentrations of Co-Cultured Endothelial Cells on Assessment of the Release of Lactate Dehydrogenase (LDH)
07:36Conclusion

Summary

Tadução automática

空间距离是评估分离内皮和心肌细胞层共培养模型中缺氧/再氧合损伤的关键参数，首次表明优化共培养空间环境对于为测试内皮细胞在心肌细胞保护中的作用提供有利的体外模型是必要的。

Tags

Cell Co-culture Cardiac Ischemia/reperfusion In Vitro Model Cardiomyocyte Endothelial Cell Spatial Environment Cell-cell Interactions Hypoxia Reperfusion Injury Cell Culture Cell Seeding Density Extracellular Matrix

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析

一个多室中枢神经系统的神经胶质细胞共培养的微流体平台

共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法

卵裂球外植体测试胚胎发育过程中细胞命运的承诺

在鼠标提睾肌为外周干细胞迁移分析的微循环活体显微镜

对缺血/再灌注在缺氧，饥饿型号<em> C。线虫</em

隔离成年小鼠心肌细胞的细胞信号传导和文化及<em>在体外</em>心肌肥厚

在体外合成在人体细胞修饰蛋白表达的mRNA的感应

小鼠模型的验证扰乱LINC配合物在细胞特异性的方式

在细胞间接触缺失的两个蜂窝型的插入共培养系统的开发

Read Article