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Генерация органоидов мозга из индуцированных плюрипотентных стволовых клеток в самодельных мини-биореакторах
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1Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 4Department of Pathological Anatomy,North-Western State Medical University, 5Department of Biology,Lomonosov State University
Chapters
- 00:02Introduction
- 01:41Transforming Petri Dishes Into Mini Bioreactors
- 02:51Induction of Neuronal Differentiation of iPSCs
- 03:51The Formation of Spheroids from Neuroepithelial Precursor Cells
- 05:38Obtaining and Cultivation of Organoids
- 07:59Results
- 09:19Conclusion
Tags
Brain OrganoidInduced Pluripotent Stem CellsMini Bioreactors3D ModelCentral Nervous System PathologiesRegenerative MedicineDifferentiationShakerLow-adhesionBioreactorsNutrient MediumPlastic CrumbsChloroformLiquid PlasticPetri DishPluripotent Stem CellsDulbecco Modified Eagle MediumFisher-12 MediumSerum Replacement
