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体外使用表达β半乳糖苷酶的寄生虫针对 克氏锥虫 的所有生命周期阶段进行药物筛选

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

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体外使用表达β半乳糖苷酶的寄生虫针对克氏锥虫的所有生命周期阶段进行药物筛选

DOI:

10.3791/63210-v

•

08:48 min

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November 05, 2021

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Victoria Lucia Alonso², Romina Manarin, Virginia Perdomo, Ernesto Gulin, Esteban Serra², Pamela Cribb²

¹Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario (UNR), ²Instituto de Biología Molecular y Celular de Rosario (IBR),CONICET, ³Instituto de Investigaciones Biomédicas (INBIOMED),Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

Capítulos

00:04Introduction
00:38Trypanosoma cruzi Epimastigotes Culture Preparation
01:58Trypanosoma cruzi Amastigote Culture Preparation
03:09Trypanosoma cruzi Trypomastigote Culture Preparation
04:50β-Galactosidase Assay
07:59Results: Results of β-Galactosidase Assay
08:23Conclusion

Summary

Tadução automática

我们描述了一种高通量比色测定法，用于测量恰加斯病病原体 克氏锥虫三个生命周期阶段的β半乳糖苷酶活性。该测定可用于以简单，快速和可重复的方式鉴定杀锥虫化合物。

Tags

Trypanosoma Cruzi In Vitro Drug Screening High-throughput Colorimetric Assay Parasite Life Cycle Stages Epimastigotes Trypomastigotes Vero Cells Beta-galactosidase Chagas Disease Drug Discovery

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