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蛍光標識タンパク質と人工リン脂質マイクロベシクルの定量的フローサイトメトリーによる相互作用の解析
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
DOI:

08:26 min

April 06, 2022

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Chapters

  • 00:04Introduction
  • 00:58Kinetic Binding Experiments
  • 02:13Equilibrium Binding Experiments
  • 02:45Analysis of Flow Cytometry Data
  • 05:13Converting Fluorescence Intensity to the Mean Number of Binding Sites
  • 07:05Results: Specific Binding of Fluorescent-Labeled Coagulation Factor X or fX to Artificial Phospholipid Vesicles
  • 07:42Conclusion

Summary

Automatic Translation

ここでは、タンパク質と細胞または微小小胞の膜との相互作用を特徴付けるための一連の方法について説明する。

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