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胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养

DOI:
10.3791/65069-v

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07:44 min

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March 03, 2023

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, , ,
1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences,University of Lisbon

Capítulos

  • 00:04Introduction
  • 01:12Fetus Tissue Excision
  • 02:12Fetal Skeletal Muscle Decellularization
  • 03:38Cell Culture in Decellularized Matrices
  • 05:24Results: Fetal Muscle Tissue Protein Analysis
  • 07:16Conclusion

Summary

Tadução automática

Tadução automática

在这项工作中，优化了脱细胞方案以获得胎鼠骨骼肌的脱细胞基质。C2C12成肌细胞可以在这些基质中定植，增殖和分化。这种 体外 模型可用于研究骨骼肌疾病（如肌肉萎缩症）背景下的细胞行为。

Tags

3D Decellularized MatricesFetal Mouse Skeletal MuscleCell CultureLAMA2-CMDCongenital Muscle DystrophyExtracellular MatrixDevelopmental ModelProtocolDecellularizationWashingStorage

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