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产生稳定的转基因C.使用微量注射线虫
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Biologia
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JoVE Journal Biologia
Generation of Stable Transgenic C. elegans Using Microinjection
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产生稳定的转基因C.使用微量注射线虫

DOI:
10.3791/833-v

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12:09 min

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August 15, 2008

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, , ,
1Department of Biological Sciences,University of Alabama

Capítulos

  • 00:00Título
  • 00:47Introduction
  • 01:31Preparing for Microinjection
  • 04:38Setting Up the Microinjection Microscope
  • 07:16Injecting Expression Vector Constructs into C. elegans
  • 10:31Screening for Transgenic C. elegans
  • 11:41Conclusion

Summary

Tadução automática

Tadução automática

本视频演示，到线虫的性腺创建转基因动物的显微注射技术。

Tags

Transgenic C. ElegansMicroinjectionDNA PlasmidConcatamersPhenotypic MarkerGFPCellular Localization StudiesGene ExpressionGermlineEarly EmbryoExperimental ParadigmsTransgenic WormsSelectionMaintenance

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