Journal
/
/
Calcium Imaging of Cortical Neurons using Fura-2 AM
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Calcium Imaging of Cortical Neurons using Fura-2 AM
DOI:

12:15 min

January 19, 2009

,

Capítulos

  • 00:00Título
  • 00:53Introduction
  • 01:15Loading Cells with Fura-2 Calcium Indicator Dye
  • 05:02Calcium Imaging of Cells
  • 09:27Defining and Analyzing Regions of Interest (ROI)
  • 10:29Representative Results/Outcome
  • 11:30Conclusion

Summary

Tadução automática

Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.

Vídeos Relacionados

Read Article