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Capítulos
- 00:00Título
- 00:53Introduction
- 01:50Preparing The Peptides
- 03:32Solubilizing and Photo-Cross-Linking the HFIP-Treated Peptide
- 06:22Representative Result
- 07:54Conclusion
Photo-geïnduceerde verknoping van niet-gemodificeerde eiwitten (PICUP) maakt karakterisering van oligomeer grootte van de distributie in metastabiele eiwitmengsels. Tonen we de toepassing van PICUP aan drie representatieve amyloidogenic peptiden van de 40 - en 42-residu vormen van amyloid β-eiwit, en calcitonine, en een controle peptide groei-hormoon releasing factor.
Tags
Photo-Induced Cross-Linking Of Unmodified ProteinsPICUPAmyloidogenic PeptidesProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseHereditary Amyotrophic Lateral SclerosisType 2 DiabetesOligomer Size DistributionClassical MethodsElectrophoresisChromatographyFluorescenceDynamic Light ScatteringMetastable MixturesCovalent Cross-linkingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)Size-exclusion Chromatography (SEC)VisualizationQuantitative AnalysisAssembly MonitoringSequence ModificationsOligomerization Mechanism
