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Capítulos
- 00:00Título
- 00:53Introduction
- 01:50Preparing The Peptides
- 03:32Solubilizing and Photo-Cross-Linking the HFIP-Treated Peptide
- 06:22Representative Result
- 07:54Conclusion
Foto-inducerad bryggbindningen av omodifierade proteiner (picup) tillåter karakterisering av oligomerer storleksfördelningen i metastabila protein blandningar. Vi visar tillämpning av picup till tre representativa amyloidogenic peptider i 40 - och 42-rester former av amyloid β-protein, och kalcitonin, och en kontroll peptid tillväxt-hormone releasing faktor.
Tags
Photo-Induced Cross-Linking Of Unmodified ProteinsPICUPAmyloidogenic PeptidesProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseHereditary Amyotrophic Lateral SclerosisType 2 DiabetesOligomer Size DistributionClassical MethodsElectrophoresisChromatographyFluorescenceDynamic Light ScatteringMetastable MixturesCovalent Cross-linkingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)Size-exclusion Chromatography (SEC)VisualizationQuantitative AnalysisAssembly MonitoringSequence ModificationsOligomerization Mechanism
