Journal
/
Biologia
/
Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

DOI:
10.3791/1086-v

•

13:51 min

•

February 18, 2009

•

, , , ,
1Department of Marine Sciences,University of Georgia (UGA)

Capítulos

  • 00:00Título
  • 01:06Introduction
  • 01:51Preparation and Environmental RNA Collection
  • 03:32Total RNA Extraction
  • 06:03First rRNA Removal
  • 07:29Second rRNA Removal
  • 10:38mRNA Amplification
  • 12:01cDNA Synthesis
  • 12:51Representative Results/ Outcome
  • 13:16Conclusion

Summary

Tadução automática

Tadução automática

We present a method for generating cDNA from environmental mRNA. In general, total RNA is first collected from the environment, rRNA is selectively removed, mRNA is selectively amplified, and cDNA synthesized from the enriched mRNA pool is sequenced. Recovered sequences can be annotated using standard bioinformatics techniques to identify the expressed genes.

Tags

Gene ExpressionMarine Microbial CommunitiesEnvironmental TranscriptomicsMetatranscriptomicsMRNA SequencingProkaryotic MRNAPoly(A) TailsEukaryotic MessagesClone LibrariesRandom PrimersCDNA SynthesisLinear AmplificationIn Vitro Transcription

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Microbial Communities in Nature and Laboratory - Interview

Biology of Microbial Communities - Interview

Bacterial Gene Expression Analysis Using Microarrays

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

In vitro Reconstitution of the Active T. castaneum Telomerase

Extraction of High Molecular Weight DNA from Microbial Mats

Konsentrasjon av metabolitter fra lav tetthet Planktoniske Felles innsats for miljøet metabolomics med Nuclear Magnetic Resonance Spectroscopy

Bruke SecM Arrest sekvens som et verktøy for å isolere ribosom Innbundne polypeptider

Read Article