Journal
/
Biologia
/
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

DOI:
10.3791/1105-v

•

09:35 min

•

February 09, 2009

•

, ,
1Department of Zoology,University of British Columbia – UBC

Capítulos

  • 00:00Título
  • 00:58Introduction
  • 01:27Disruption of the Influenza A Virion
  • 02:53Glycerol Gradient Sediment Velocity Centrifugation
  • 04:18Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
  • 05:40Concentration of the vRNP Fractions
  • 06:39Negative Staining of vRNPs
  • 08:25Representative Results/ Outcome
  • 09:05Conclusion

Summary

Tadução automática

Tadução automática

The genome of the influenza A virus consists of eight separate complexes of RNA and proteins, termed viral ribonucleoprotein complexes (vRNPs). This paper describes the glycerol gradient purification and transmission electron microscopy visualization of influenza A vRNPs.

Tags

PurificationVisualizationInfluenza AViral Ribonucleoprotein ComplexesVRNPsViral EnvelopeNuclear ImportReplicationIsolated VRNPsProcedimentoDisruptionDetergentsGlycerol GradientVelocity SedimentationSDS-PAGECoomassie Blue StainingPeak FractionsConcentrationCentrifugationIntegrity VerificationTransmission Electron Microscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Purification of Hsp104, a Protein Disaggregase

Chromatografisk Rensning af højaktiv Gær ribosomer

Fremgangsmåde til isolering og identifikation af mRNA'er, microRNA og proteinkomponenter af ribonucleoprotein komplekser fra celleekstrakter ved hjælp af RIP-Chip

Identifikation af protein-komplekser i<em> Escherichia coli</em> Hjælp Sekventiel Peptide Affinitetsrensning i kombination med Tandem massespektrometri

AFM-baserede Kortlægning af de elastiske egenskaber af cellevægge: ved Tissue, Cellular, og Subcellulære resolutioner

Et-trins Oprensning af Twin-Strep-mærkede proteiner og deres komplekser på Strep-Tactin Resin tværbundet med Bis (sulfosuccinimidyl) suberat (BS3)

Hurtig isolering og oprensning af mitokondrier til transplantation Ved Tissue Dissociation og differentieret Filtrering

Generation og oprensning af human INO80 Chromatin Remodeling komplekser og Subcomplexes

Read Article