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Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae
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Biologia
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JoVE Journal Biologia
Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

DOI:
10.3791/1109-v

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05:46 min

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February 06, 2009

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1Department of Physiology and Cellular Biophysics,Columbia University College of Physicians and Surgeons

Capítulos

  • 00:00Título
  • 00:41Introduction
  • 01:06Dissection of Drosophila Larvae
  • 01:56Intracellular recordings from larval muscle cells
  • 04:51Representative Results/Outcome
  • 05:06Conclusion

Summary

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Here we describe electrophysiological methods for measuring synaptic transmission at the neuromuscular junction of Drosophila larva. Evoked release is initiated artificially by stimulating the motor neuron axons, and transmission through the NMJ can be measured by the postsynaptic response evoked in the muscle.

Tags

Electrophysiological MethodsRecording Synaptic PotentialsNMJDrosophila LarvaeNeuromuscular JunctionSynaptic TransmissionLarval Neuromuscular SystemSynaptic PhysiologyNeurotransmissionExperimental ManipulationIntracellular RecordingMuscle InnervationTransilluminationExcitatory Junction PotentialsGlutamate ReleaseIntracellular ElectrodeAction Potentials

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