É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:54Introduction
- 01:29Preparation of RNA
- 03:26Preparation of Morpholino
- 05:07Preparing the Micropipette and Plates for Microinjections
- 07:51Preparing and Injecting Fertilized Zebrafish Embryos
- 10:09Representative Results: EGFP Expression and Mutant Phenotypes
- 11:07Conclusion
Tags
MicroinjectionMRNAMorpholino Antisense OligonucleotidesZebrafish EmbryosGene KnockdownOverexpression StudiesMisexpression StudiesGene FunctionIn Vivo AssayEGFP MRNATranslational-blocking Morpholino OligoAutosomal Dominant Polycystic Kidney Disease (ADPKD)1-cell Stage EmbryosGFP ExpressionPhenotype AnalysisTransgenic AnimalsGerm-line ChimerasCell-fate MappingGene Screening
