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Biologia
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Microinjection of Zebrafish Embryos to Analyze Gene Function
JoVE Journal
Biologia
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JoVE Journal Biologia
Microinjection of Zebrafish Embryos to Analyze Gene Function

Microinjection of Zebrafish Embryos to Analyze Gene Function

DOI:
10.3791/1115-v

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07:18 min

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March 09, 2009

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, ,
1Department of Genetics,Harvard Medical School, 2Department of Cardiology,Children’s Hospital Boston

Capítulos

  • 00:00Título
  • 00:42Introduction
  • 01:12Zebrafish Egg Collection
  • 02:24Needle Pulling, Loading, and Preparation
  • 04:23Injection
  • 05:48Representative Results: Morpholino and mRNA Injection Phenotypes
  • 06:33Conclusion

Summary

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This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.

Tags

MicroinjectionZebrafish EmbryosGene Function AnalysisProtein ManipulationMorpholinosAntisense OligonucleotidesGene ExpressionMRNA InjectionMicroinjection ProcessEmbryo ManipulationInjection Volume

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