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Measuring Caenorhabditis elegans Life Span on Solid Media
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Biologia
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JoVE Journal Biologia
Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

DOI:
10.3791/1152-v

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12:49 min

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May 12, 2009

•

,
1Department of Pathology,University of Washington, 2Molecular and Cellular Biology Program,University of Washington

Capítulos

  • 00:00Título
  • 01:06Introduction
  • 01:28Prepare Nematode Growth Media (NGM) Plates
  • 05:27Age-Synchronizing a Population of Animals
  • 08:20Score Animals for Life Span
  • 11:41Representative Results: Life Span Analysis
  • 12:18Conclusion

Summary

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In this article we present a general protocol for measuring life span of nematodes maintained on solid media with UV-killed bacterial food.

Tags

Caenorhabditis ElegansLife Span MeasurementSolid MediaAgingCellular DeteriorationOrganelle DeteriorationMortalityModel OrganismBiology Of AgingFunctional DeclineTotal Rate Of AgingNematodesResponsiveness To External StimuliLiquid MediaPlatesUV-killed Bacteria DietProtocolNematode Growth Media

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